New restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus
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منابع مشابه
Restriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
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In order to study polymorphisms of the DNA insertion sequence 6110 (IS6110) in Mycobacterium tuberculosis strains isolated from Colombian patients, together with resistance to antituberculous medications in the Department of Quindío, Colombia, a prospective study was conducted using a consecutive sample of 59 patients with symptomatic pulmonary tuberculosis whose cases had been confirmed by bac...
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A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...
متن کاملExercise 21 RESTRICTION ENDONUCLEASE DIGESTION OF DNA AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)
Present-day DNA technology is partially dependent upon the ability of investigators to cut DNA molecules at specific sites with enzymes called restriction endonucleases or restriction enzymes. The most useful of these enzymes, which occur naturally within bacteria, recognize particular "target" or recognition sequences (restriction sites) within a DNA double helix, and catalyze the cleavage of ...
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Recent development of DNA markers provides powerful tools for population genetic analyses. Amplified fragment length polymorphism (AFLP) markers result from a polymerase chain reaction (PCR)-based DNA fingerprinting technique that can detect multiple restriction fragments in a single polyacrylamide gel, and thus are potentially useful for population genetic studies. Because AFLP markers have to...
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ژورنال
عنوان ژورنال: FEMS Immunology and Medical Microbiology
سال: 2001
ISSN: 0928-8244
DOI: 10.1016/s0928-8244(01)00232-2